Transmission confocal laser scanning microscopy with a virtual pinhole based on nonlinear detection.

نویسندگان

  • C Yang
  • J Mertz
چکیده

We present a transmission-mode confocal laser scanning microscope system based on the use of second-harmonic generation (SHG) for signal detection. Our method exploits the quadratic intensity dependence of SHG to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal acts as a virtual pinhole that remains self-aligned without the need for descanning.

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عنوان ژورنال:
  • Optics letters

دوره 28 4  شماره 

صفحات  -

تاریخ انتشار 2003